Coupled amplification and sequencing of genomic DNA.
Addition of dideoxyribonucleotides during the exponential phase of the PCR should result in the synthesis of two complementary sequence ladders. We have explored this hypothesis to develop coupled amplification and sequencing of genomic DNA. Coupled amplification and sequencing is a biphasic method...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51330/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1672768/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.7.2815 |
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