Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification.
We have devised a protocol to obtain accurate and complete sequences of the immunoglobulin heavy- and light-chain variable-region (VH and VL) genes of single B-hybridoma cells that express defined V genes. The amplification achieved ranges from 2 x 10(13)- to 1 x 10(14)-fold. Only one potential Taq...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49760/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1502172/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.16.7610 |
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