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Efficient generation of selection‐gene‐free rat knockout models by homologous recombination in ES cells

Embryonic stem cell (ES cell)‐based rat knockout technology, although successfully developed in 2010, has seen very limited usage to date due to low targeting efficiency and a lack of optimized procedures. In this study, we performed gene targeting in ES cells from the Sprague–Dawley (SD) and the Fi...

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書目詳細資料
發表在:FEBS Lett
Main Authors: Lan, He, Li, Shuping, Guo, Zihang, Men, Hongsheng, Wu, Yuanyuan, Li, Ning, Bryda, Elizabeth C., Capecchi, Mario R., Wu, Sen
格式: Artigo
語言:Inglês
出版: John Wiley and Sons Inc. 2016
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5129459/
https://ncbi.nlm.nih.gov/pubmed/27597178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1873-3468.12388
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