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Internal epitope tagging informed by relative lack of sequence conservation

Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by inte...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Burg, Leonard, Zhang, Karen, Bonawitz, Tristan, Grajevskaja, Viktorija, Bellipanni, Gianfranco, Waring, Richard, Balciunas, Darius
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2016
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5125009/
https://ncbi.nlm.nih.gov/pubmed/27892520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep36986
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