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Internal epitope tagging informed by relative lack of sequence conservation

Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by inte...

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Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Burg, Leonard, Zhang, Karen, Bonawitz, Tristan, Grajevskaja, Viktorija, Bellipanni, Gianfranco, Waring, Richard, Balciunas, Darius
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5125009/
https://ncbi.nlm.nih.gov/pubmed/27892520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep36986
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