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Internal epitope tagging informed by relative lack of sequence conservation

Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by inte...

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Bibliografiset tiedot
Julkaisussa:Sci Rep
Päätekijät: Burg, Leonard, Zhang, Karen, Bonawitz, Tristan, Grajevskaja, Viktorija, Bellipanni, Gianfranco, Waring, Richard, Balciunas, Darius
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Nature Publishing Group 2016
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5125009/
https://ncbi.nlm.nih.gov/pubmed/27892520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep36986
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