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Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7
Peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. However, the peptide tags can disrupt the target protein function. When function is disrupted by fusing...
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| Publicado no: | Genetics |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4423380/ https://ncbi.nlm.nih.gov/pubmed/25745023 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.114.169482 |
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