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A novel process of viral vector barcoding and library preparation enables high-diversity library generation and recombination-free paired-end sequencing

Detailed characterization and mapping of oligonucleotide function in vivo is generally a very time consuming effort that only allows for hypothesis driven subsampling of the full sequence to be analysed. Recent advances in deep sequencing together with highly efficient parallel oligonucleotide synth...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Davidsson, Marcus, Diaz-Fernandez, Paula, Schwich, Oliver D., Torroba, Marcos, Wang, Gang, Björklund, Tomas
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5118689/
https://ncbi.nlm.nih.gov/pubmed/27874090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep37563
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