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Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3–5-kb DNA fragment are not easy to identify and pose problems during mapping and de novo assembly. Longer...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3273786/ https://ncbi.nlm.nih.gov/pubmed/22127871 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1000 |
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