Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination

Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3–5-kb DNA fragment are not easy to identify and pose problems during mapping and de novo assembly. Longer...

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Bibliografiske detaljer
Main Authors: Van Nieuwerburgh, Filip, Thompson, Ryan C., Ledesma, Jessica, Deforce, Dieter, Gaasterland, Terry, Ordoukhanian, Phillip, Head, Steven R.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2012
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3273786/
https://ncbi.nlm.nih.gov/pubmed/22127871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1000
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