Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

BACKGROUND: Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compa...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Chang, Yizhao, Su, Tianyuan, Qi, Qingsheng, Liang, Quanfeng
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5109708/
https://ncbi.nlm.nih.gov/pubmed/27842593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0594-4
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