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Real-Time Detection of a Self-Replicating RNA Enzyme

A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that di...

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Pubblicato in:Molecules
Autori principali: Olea, Charles, Joyce, Gerald F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5108293/
https://ncbi.nlm.nih.gov/pubmed/27706059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules21101310
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