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Sequential and counter-selectable cassettes for fission yeast

BACKGROUND: Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of interest. In yeasts, this is achieved by PCR amplification of the cassette flank...

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Bibliografische gegevens
Gepubliceerd in:BMC Biotechnol
Hoofdauteurs: Amelina, Hanna, Moiseeva, Vera, Collopy, Laura Catharine, Pearson, Siân Rosanna, Armstrong, Christine Anne, Tomita, Kazunori
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5101803/
https://ncbi.nlm.nih.gov/pubmed/27825338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0307-4
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