Sequential and counter-selectable cassettes for fission yeast

BACKGROUND: Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of interest. In yeasts, this is achieved by PCR amplification of the cassette flank...

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Bibliografiske detaljer
Udgivet i:BMC Biotechnol
Main Authors: Amelina, Hanna, Moiseeva, Vera, Collopy, Laura Catharine, Pearson, Siân Rosanna, Armstrong, Christine Anne, Tomita, Kazunori
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5101803/
https://ncbi.nlm.nih.gov/pubmed/27825338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0307-4
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