Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predict highly effective single guide RNAs (sgRNAs) for...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Horlbeck, Max A, Gilbert, Luke A, Villalta, Jacqueline E, Adamson, Britt, Pak, Ryan A, Chen, Yuwen, Fields, Alexander P, Park, Chong Yon, Corn, Jacob E, Kampmann, Martin, Weissman, Jonathan S
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2016
Assuntos:
Computational and Systems Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5094855/
https://ncbi.nlm.nih.gov/pubmed/27661255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.19760
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