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Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates
We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl‐tRNA synthetase/tRNA(CUA) pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal...
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| Publicado no: | Chembiochem |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5094518/ https://ncbi.nlm.nih.gov/pubmed/27197715 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201600138 |
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