Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

This protocol describes how villous cytotrophoblast cells are isolated from placentas at term by successive enzymatic digestions, followed by density centrifugation, media gradient isolation and immunomagnetic purification. As observed in vivo, mononucleated villous cytotrophoblast cells in primary...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Sagrillo-Fagundes, Lucas, Clabault, Hélène, Laurent, Laetitia, Hudon-Thibeault, Andrée-Anne, Salustiano, Eugênia Maria Assunção, Fortier, Marlène, Bienvenue-Pariseault, Josianne, Wong Yen, Philippe, Sanderson, J. Thomas, Vaillancourt, Cathy
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2016
Soggetti:
Developmental Biology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5091709/
https://ncbi.nlm.nih.gov/pubmed/27500522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54228
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