Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

This protocol describes how villous cytotrophoblast cells are isolated from placentas at term by successive enzymatic digestions, followed by density centrifugation, media gradient isolation and immunomagnetic purification. As observed in vivo, mononucleated villous cytotrophoblast cells in primary...

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Veröffentlicht in:J Vis Exp
Hauptverfasser: Sagrillo-Fagundes, Lucas, Clabault, Hélène, Laurent, Laetitia, Hudon-Thibeault, Andrée-Anne, Salustiano, Eugênia Maria Assunção, Fortier, Marlène, Bienvenue-Pariseault, Josianne, Wong Yen, Philippe, Sanderson, J. Thomas, Vaillancourt, Cathy
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2016
Schlagworte:
Developmental Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5091709/
https://ncbi.nlm.nih.gov/pubmed/27500522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54228
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