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Substrate and Cofactor Dynamics on Guanosine Monophosphate Reductase Probed by High Resolution Field Cycling (31)P NMR Relaxometry

Guanosine-5′-monophosphate reductase (GMPR) catalyzes the reduction of GMP to IMP and ammonia with concomitant oxidation of NADPH. Here we investigated the structure and dynamics of enzyme-bound substrates and cofactors by measuring (31)P relaxation rates over a large magnetic field range using high...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Rosenberg, Masha M., Redfield, Alfred G., Roberts, Mary F., Hedstrom, Lizbeth
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5087720/
https://ncbi.nlm.nih.gov/pubmed/27613871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.739516
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