The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment

OBJECTIVE: CRISPR/Cas9 technology provides a powerful tool for targeted modification of genomes. In this system, a donor DNA harboring two flanking homology arms is mostly used for targeted insertion of long exogenous DNA. Here, we introduced an alternative design for the donor DNA by incorporation...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Cell J
Autores principales: Basiri, Mohsen, Behmanesh, Mehrdad, Tahamtani, Yaser, Khalooghi, Keynoosh, Moradmand, Azadeh, Baharvand, Hossein
Formato: Artigo
Lenguaje:Inglês
Publicado: Royan Institute 2017
Materias:
Original Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5086331/
https://ncbi.nlm.nih.gov/pubmed/28042537
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares