The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment

OBJECTIVE: CRISPR/Cas9 technology provides a powerful tool for targeted modification of genomes. In this system, a donor DNA harboring two flanking homology arms is mostly used for targeted insertion of long exogenous DNA. Here, we introduced an alternative design for the donor DNA by incorporation...

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Détails bibliographiques
Publié dans:Cell J
Auteurs principaux: Basiri, Mohsen, Behmanesh, Mehrdad, Tahamtani, Yaser, Khalooghi, Keynoosh, Moradmand, Azadeh, Baharvand, Hossein
Format: Artigo
Langue:Inglês
Publié: Royan Institute 2017
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Original Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5086331/
https://ncbi.nlm.nih.gov/pubmed/28042537
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