Location of the active site for enzyme-adenylate formation in DNA ligases.

The enzyme-AMP reaction intermediate of the 102-kDa bovine DNA ligase I was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the acid-labile phosphoramidate bond. Microsequencing of the peptide showed that it contains an internal tryps...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Tomkinson, A E, Totty, N F, Ginsburg, M, Lindahl, T
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1991
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50818/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1988940/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.2.400
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