Location of the active site for enzyme-adenylate formation in DNA ligases.
The enzyme-AMP reaction intermediate of the 102-kDa bovine DNA ligase I was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the acid-labile phosphoramidate bond. Microsequencing of the peptide showed that it contains an internal tryps...
Zapisane w:
| Wydane w: | Proc Natl Acad Sci U S A |
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| Główni autorzy: | , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
National Academy of Sciences
1991
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50818/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1988940/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.2.400 |
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