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Pairing Beyond the Seed Supports MicroRNA Targeting Specificity

To identify endogenous miRNA target sites, we isolated AGO-bound RNAs from Caenorhabditis elegans by individual-nucleotide resolution crosslinking-immunoprecipitation, which fortuitously also produced miRNA-target chimeric reads. Through the analysis of thousands of reproducible chimeras, pairing to...

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Détails bibliographiques
Publié dans:Mol Cell
Auteurs principaux: Broughton, James P., Lovci, Michael T., Huang, Jessica L., Yeo, Gene W., Pasquinelli, Amy E.
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5074850/
https://ncbi.nlm.nih.gov/pubmed/27720646
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2016.09.004
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