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In vitro Enzymology of Cas9
Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms. Here, we describe protocols...
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5074358/ https://ncbi.nlm.nih.gov/pubmed/25398333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801185-0.00001-5 |
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