A novel methanol-free Pichia pastoris system for recombinant protein expression

BACKGROUND: As one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P(AOX1)) which is strongly induced by methanol. However, the toxic and inflammatory nature of methanol restricts its application, especially in edible and medical...

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Detalles Bibliográficos
Publicado en:Microb Cell Fact
Main Authors: Shen, Wei, Xue, Ying, Liu, Yiqi, Kong, Chuixing, Wang, Xiaolong, Huang, Mengmeng, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2016
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Research
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5073731/
https://ncbi.nlm.nih.gov/pubmed/27769297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0578-4
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