A novel methanol-free Pichia pastoris system for recombinant protein expression

BACKGROUND: As one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P(AOX1)) which is strongly induced by methanol. However, the toxic and inflammatory nature of methanol restricts its application, especially in edible and medical...

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Bibliografiske detaljer
Udgivet i:	Microb Cell Fact
Main Authors:	Shen, Wei, Xue, Ying, Liu, Yiqi, Kong, Chuixing, Wang, Xiaolong, Huang, Mengmeng, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Format:	Artigo
Sprog:	Inglês
Udgivet:	BioMed Central 2016
Fag:	Research
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC5073731/ https://ncbi.nlm.nih.gov/pubmed/27769297 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0578-4
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A novel methanol-free Pichia pastoris system for recombinant protein expression

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