A novel methanol-free Pichia pastoris system for recombinant protein expression

BACKGROUND: As one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P(AOX1)) which is strongly induced by methanol. However, the toxic and inflammatory nature of methanol restricts its application, especially in edible and medical...

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Bibliografske podrobnosti
izdano v:Microb Cell Fact
Main Authors: Shen, Wei, Xue, Ying, Liu, Yiqi, Kong, Chuixing, Wang, Xiaolong, Huang, Mengmeng, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2016
Teme:
Research
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5073731/
https://ncbi.nlm.nih.gov/pubmed/27769297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0578-4
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