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Genomic Disruption of VEGF-A Expression in Human Retinal Pigment Epithelial Cells Using CRISPR-Cas9 Endonuclease
PURPOSE: To employ type II clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 endonuclease to suppress ocular angiogenesis by genomic disruption of VEGF-A in human RPE cells. METHODS: CRISPR sequences targeting exon 1 of human VEGF-A were computationally identified based on pred...
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| Publicado en: | Invest Ophthalmol Vis Sci |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The Association for Research in Vision and Ophthalmology
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5072539/ https://ncbi.nlm.nih.gov/pubmed/27768202 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.16-20296 |
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