Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we have achieved > 95% replacement of tryptophan in the repressor. We show that the absorbance and fl...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, Laws, W R, Hasselbacher, C A
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC50690/
https://ncbi.nlm.nih.gov/pubmed/1465434
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados