Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we have achieved > 95% replacement of tryptophan in the repressor. We show that the absorbance and fluor...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, Laws, W R, Hasselbacher, C A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50690/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1465434/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.24.12023
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