Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we have achieved > 95% replacement of tryptophan in the repressor. We show that the absorbance and fl...

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Autori principali: Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, Laws, W R, Hasselbacher, C A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC50690/
https://ncbi.nlm.nih.gov/pubmed/1465434
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