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Purification of bacteriophage lambda repressor

Bacteriophage lambda repressor controls the lysogeny/lytic growth switch after infection of E. coli by lambda phage. In order to study in detail the looping of DNA mediated by the protein, tag-free repressor and a loss-of-cooperativity mutant were expressed in E.coli and purified by (1) ammonium sul...

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Detalhes bibliográficos
Main Authors: Gao, Ning, Shearwin, Keith, Mack, John, Finzi, Laura, Dunlap, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3761395/
https://ncbi.nlm.nih.gov/pubmed/23831434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.06.013
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