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Transcriptionally competent chromatin assembled with exogenous histones in a yeast whole cell extract
We describe a cell-free chromatin assembly system derived from the yeast Saccharomyces cerevisiae, which efficiently packages DNA into minichromosomes in a reaction dependent on exogenous core histones and an ATP-regenerating system. Both supercoiled and relaxed plasmid DNA serve as templates for nu...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC506827/ https://ncbi.nlm.nih.gov/pubmed/15282330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh107 |
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