Transcriptionally competent chromatin assembled with exogenous histones in a yeast whole cell extract

We describe a cell-free chromatin assembly system derived from the yeast Saccharomyces cerevisiae, which efficiently packages DNA into minichromosomes in a reaction dependent on exogenous core histones and an ATP-regenerating system. Both supercoiled and relaxed plasmid DNA serve as templates for nu...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Rodríguez-Campos, Antonio, Koop, Ronald, Faraudo, Susanna, Beato, Miguel
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2004
Fag:	NAR Methods Online
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC506827/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15282330/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh107
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