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Changing the recognition specificity of a DNA-methyltransferase by in vitro evolution
The gene coding for the SinI DNA-methyltransferase, a modification enzyme able to recognize and methylate the internal cytosine of the GG(A)/(T)CC sequence, was subjected to in vitro mutagenesis, DNA-shuffling and a strong selection for relaxed GGNCC recognition specificity. As a result of this in v...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2004
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC506809/ https://ncbi.nlm.nih.gov/pubmed/15273276 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh724 |
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