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Changing the recognition specificity of a DNA-methyltransferase by in vitro evolution

The gene coding for the SinI DNA-methyltransferase, a modification enzyme able to recognize and methylate the internal cytosine of the GG(A)/(T)CC sequence, was subjected to in vitro mutagenesis, DNA-shuffling and a strong selection for relaxed GGNCC recognition specificity. As a result of this in v...

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Detalhes bibliográficos
Main Authors: Tímár, Edit, Groma, Gergely, Kiss, Antal, Venetianer, Pál
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC506809/
https://ncbi.nlm.nih.gov/pubmed/15273276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh724
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