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A proximity-dependent assay for specific RNA–protein interactions in intact cells

The proximity ligation assay (PLA) is an immune staining method that detects protein–protein interactions in fixed cells. We describe here RNA–PLA, a simple adaptation of this technology that allows the detection of specific RNA–protein interactions in fixed cells by using a DNA oligonucleotide that...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Zhang, Wei, Xie, Mingyi, Shu, Mei-Di, Steitz, Joan A., DiMaio, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5066630/
https://ncbi.nlm.nih.gov/pubmed/27659050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.058248.116
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