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Characterizing Active Site Conformational Heterogeneity along the Trajectory of an Enzymatic Phosphoryl Transfer Reaction

States along the phosphoryl transfer reaction catalyzed by the nucleoside monophosphate kinase UmpK were captured and changes in the conformational heterogeneity of conserved active site arginine side‐chains were quantified by NMR spin‐relaxation methods. In addition to apo and ligand‐bound UmpK, a...

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Библиографические подробности
Опубликовано в: :Angew Chem Int Ed Engl
Главные авторы: Zeymer, Cathleen, Werbeck, Nicolas D., Zimmermann, Sabine, Reinstein, Jochen, Hansen, D. Flemming
Формат: Artigo
Язык:Inglês
Опубликовано: John Wiley and Sons Inc. 2016
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5026167/
https://ncbi.nlm.nih.gov/pubmed/27534930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201606238
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