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Dual randomization of oligonucleotides to reduce the bias in ribosome-profiling libraries

Protein translation is at the heart of cellular metabolism and its in-depth characterization is key for many lines of research. Recently, ribosome profiling became the state-of-the-art method to quantitatively characterize translation dynamics at a transcriptome-wide level. However, the strategy of...

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Publicado en:Methods
Autores principales: Lecanda, Aarón, Nilges, Benedikt S., Sharma, Puneet, Nedialkova, Danny D., Schwarz, Juliane, Vaquerizas, Juan M., Leidel, Sebastian A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Academic Press 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5024760/
https://ncbi.nlm.nih.gov/pubmed/27450428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.07.011
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