Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe.

A synthetic oligodeoxynucleotide sequence complementary to the mRNA for the adrenocorticotrophin (ACTH) precursor pro-opiomelanocortin (POMC) was end labelled using digoxigenin. The probe was used to detect POMC mRNA both on nitrocellulose filters and by non-isotopic in situ hybridisation (NISH) in...

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Main Authors: Farquharson, M, Harvie, R, McNicol, A M
Format: Artigo
Sprog:Inglês
Udgivet: 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC502456/
https://ncbi.nlm.nih.gov/pubmed/2370311
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