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Detection of mRNA in Streptomyces Cells by Whole-Cell Hybridization with Digoxigenin-Labeled Probes

Detection of mRNA of the thiostrepton resistance gene (tsr) harbored by plasmid pIJ673 in Streptomyces violacelatus was achieved by whole-cell hybridization with digoxigenin-labeled in vitro transcripts followed by an antibody-alkaline phosphatase detection of the digoxigenin reporter molecule. Prio...

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Dettagli Bibliografici
Autori principali: Hahn, Dittmar, Amann, Rudolf I., Zeyer, Josef
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1993
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC182355/
https://ncbi.nlm.nih.gov/pubmed/16349031
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