Detection of mRNA in Streptomyces Cells by Whole-Cell Hybridization with Digoxigenin-Labeled Probes

Detection of mRNA of the thiostrepton resistance gene (tsr) harbored by plasmid pIJ673 in Streptomyces violacelatus was achieved by whole-cell hybridization with digoxigenin-labeled in vitro transcripts followed by an antibody-alkaline phosphatase detection of the digoxigenin reporter molecule. Prio...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Hahn, Dittmar, Amann, Rudolf I., Zeyer, Josef
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182355/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.8.2753-2757.1993
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