Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

A simple, efficient, and sensitive technique has been developed for amplification of cDNAs encoding molecules with 5' regions of unknown sequence. In this ligation-anchored PCR, T4 RNA ligase is used to covalently link an "anchor" oligonucleotide to first-strand cDNAs. These anchored cDNAs are then...

全面介紹

書目詳細資料

發表在:	Proc Natl Acad Sci U S A
Principais autores:	Troutt, A B, McHeyzer-Williams, M G, Pulendran, B, Nossal, G J
格式:	Artigo
語言:	Inglês
出版:	National Academy of Sciences 1992
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50225/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.20.9823
標簽:	添加標簽 沒有標簽, 成為第一個標記此記錄!