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Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

A simple, efficient, and sensitive technique has been developed for amplification of cDNAs encoding molecules with 5' regions of unknown sequence. In this ligation-anchored PCR, T4 RNA ligase is used to covalently link an "anchor" oligonucleotide to first-strand cDNAs. These anchored cDNAs are then...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Troutt, A B, McHeyzer-Williams, M G, Pulendran, B, Nossal, G J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50225/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.20.9823
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