Ligation-anchored PCR: a simple amplification technique with single-sided specificity.
A simple, efficient, and sensitive technique has been developed for amplification of cDNAs encoding molecules with 5' regions of unknown sequence. In this ligation-anchored PCR, T4 RNA ligase is used to covalently link an "anchor" oligonucleotide to first-strand cDNAs. These anchored cDNAs are then...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50225/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.20.9823 |
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