Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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Bibliografske podrobnosti
izdano v:Sensors (Basel)
Main Authors: Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2016
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017477/
https://ncbi.nlm.nih.gov/pubmed/27548185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s16081312
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