Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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Detalles Bibliográficos
Publicado en:Sensors (Basel)
Main Authors: Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro
Formato: Artigo
Idioma:Inglês
Publicado: MDPI 2016
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017477/
https://ncbi.nlm.nih.gov/pubmed/27548185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s16081312
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