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Antibody-coupled magnetic beads can be re-used in immuno-MRM assays to reduce cost and extend antibody supply

Immuno-affinity enrichment of peptides coupled to targeted, multiple reaction monitoring-mass spectrometry (immuno-MRM) enables precise quantification of peptides. Affinity-purified polyclonal antibodies are routinely used as affinity reagents in immuno-MRM assays, but they are not renewable, limiti...

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Bibliografiske detaljer
Udgivet i:J Proteome Res
Main Authors: Zhao, Lei, Whiteaker, Jeffrey R., Voytovich, Uliana J., Ivey, Richard G., Paulovich, Amanda G.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017239/
https://ncbi.nlm.nih.gov/pubmed/26302155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.5b00290
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