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Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR...
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| Udgivet i: | Nat Commun |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5007326/ https://ncbi.nlm.nih.gov/pubmed/27558837 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms12617 |
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