Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction of off-target mutations are the most important targets for optimization. Here, we describe an efficient genome editing method for bread wheat using...

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Publicado no:Nat Commun
Main Authors: Liang, Zhen, Chen, Kunling, Li, Tingdong, Zhang, Yi, Wang, Yanpeng, Zhao, Qian, Liu, Jinxing, Zhang, Huawei, Liu, Cuimin, Ran, Yidong, Gao, Caixia
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253684/
https://ncbi.nlm.nih.gov/pubmed/28098143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14261
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