Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pai...

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Hauptverfasser: Shizuya, H, Birren, B, Kim, U J, Mancino, V, Slepak, T, Tachiiri, Y, Simon, M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1992
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC50007/
https://ncbi.nlm.nih.gov/pubmed/1528894
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