In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR.
The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental procedure. Not surprisingly, conventional cloning...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science (PLoS)
2017-01-01
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| Collection: | PLoS ONE |
| Accès en ligne: | http://europepmc.org/articles/PMC5570364?pdf=render |
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