Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pai...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Shizuya, H, Birren, B, Kim, U J, Mancino, V, Slepak, T, Tachiiri, Y, Simon, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1992
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50007/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1528894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.18.8794
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